Question

In preparing a demonstration for her immunology class,

an instructor purified IgG antibodies to sheep red blood

cells (SRBCs) and digested some of the antibodies into

Fab, Fc, and F(ab.)2 fragments. She placed each prepara-

tion in a separate tube, labeled the tubes with a water-

soluble marker, and left them in an ice bucket. When the

instructor returned for her class period, she discovered

that the labels had smeared and were unreadable. Deter-

mined to salvage the demonstration, she relabeled the

tubes 1, 2, 3, and 4 and proceeded. Based on the test results

described below, indicate which preparation was con-

tained in each tube and explain how you identified the

contents.

a. The preparation in tube 1 agglutinated SRBCs but did

not lyse them in the presence of complement.

b. The preparation in tube 2 did not agglutinate SRBCs or

lyse them in the presence of complement. However, when

this preparation was added to SRBCs before the addition

of whole anti-SRBC, it prevented agglutination of the

cells by the whole anti-SRBC antiserum.

c. The preparation in tube 3 agglutinated SRBCs and also

lysed the cells in the presence of complement.​

Answer 1

Answer:

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Explanation: