in suspension culture agitation of medium serves all the purpose except
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Constant agitation at 100- 250 rpm serves the purpose of aeration, mixing and prevention of aggregation. This suspension culture is sub-cultured every week. Suspension cultures grow here more or less need to be sub-cultured rapidly than the callus culture
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For the preparation of suspension culture, callus is transferred to a liquid nutrient medium and is agitated. Cells of callus are separated due to agitation. Constant agitation at 100- 250 rpm serves the purpose of aeration, mixing and prevention of aggregation. This suspension culture is sub-cultured every week. Suspension cultures grow here more or less need to be sub-cultured rapidly than the callus culture.
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