In the experiment of preparing a temporary mount of a leaf peel to observe stomata we use glycerine and safranine . Why?
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Glycerin is used to remove the over stain of the leaf peal
Safranin is used to make the stomata of leaf peal visible clearly under microscope
Safranin is dark red in colour but ovet staining shoukd not be done
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In the experiment of temporary mount of a leaf peel (basically to view stomatal pores), the importance of. :-
◆ Glycerine :- • Providing moisture to
leaf peel, so that it don't dry up.
◆ Safranine Stain :- • Darkens the cell
wall and cell content, so that it
becomes easier to observe the
stomatal pores.
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