In which chromatography technique, molecules are separated on the basis their size and shape ?
Answers
Column chromatography is one of the most common methods of protein purification. Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.
Size-exclusion chromatography (SEC), commonly known as molecular sieve chromatography, is a chromatographic technique that separates molecules in solution based on their size and, in certain situations, their molecular mass. Large molecules or biological macromolecules, such as proteins and industrial polymers, are commonly subjected to this method. The technique is known as gel-filtration chromatography when an aqueous phase is used to convey the sample through the column, as opposed to gel permeation chromatography when an organic solvent is used as the mobile phase.