Question

indirect immunofluorescence involves fluorescently labelled ?​

Answer 1

Answer:

​​Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC). ... Indirect IF uses two antibodies.

Explanation:

Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC). Antibodies that are chemically conjugated to fluorophores are commonly used in IF.

​The fluorophore allows visualization of the target distribution in the sample under a fluorescent microscope (eg epifluorescence and confocal microscopes). We distinguish between two IF methods depending on whether the fluorophore is conjugated to the primary or the secondary antibody: