Induced mutations on the molecular level can be caused by: chemicals hydroxylamine base analogs (e.g., bromodeoxyuridine (brdu)) alkylating agents (e.g., nethylnnitrosourea (enu)). these agents can mutate both replicating and nonreplicating dna. in contrast, a base analog can mutate the dna only when the analog is incorporated in replicating the dna. each of these classes of chemical mutagens has certain effects that then lead to transitions, transversions, or deletions. agents that form dna adducts (e.g., ochratoxin a)[31] dna intercalating agents (e.g., ethidium bromide) dna crosslinkers oxidative damage nitrous acid converts amine groups on a and c to diazo groups, altering their hydrogen bonding patterns, which leads to incorrect base pairing during replication
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Mutations are caused by environmental factors known as mutagens. Types of mutagens include radiation, chemicals, and infectious agents. Mutations may be spontaneous in nature.
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