Question

Isolation and purification of enzymes biology discussion

Answer 1

Answer:

Extraction Methods: The first step in the isolation of enzymes is their extraction. Techniques that fall into this group are employed either to separate enzymes from solid substrate culture or to release enzymes from the interior of microbial cells.

Answer 2

The initial step in the "isolation of enzymes" is their extraction. Techniques that down into this group are "employed either to free enzymes" from 'solid substrate culture' or to discharge enzymes from the "interior of microbial cells".

Explanation:

• Enzyme isolation is mostly achieved using immobilized inhibitors, co-factors, dyes or other suitable ligands, or magnetic beads prepared from affinity bio-polymers are used.
• Enzyme purification is usually accomplished through the addition of a minute epitope tag to the destination protein followed by isolation through the solid-state purification using an desirable column.

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