Question

Joe denied being the father of Lisa. Lisa's mom took Joe to court to prove he is Lisa's biological father. The way to prove this would be by _____.
recombinant DNA
hybridization
DNA profiling
cloning

Answer 1

The correct answer is DNA profiling.

DNA profiling or DNA fingerprinting is a technique in forensic science which allows us to check the DNA pattern of an individual which is as unique as fingerprints.

With the help of DNA fingerprinting, Lisa's mother would be able to prove that Joe is Lisa's biological father.

Answer 2

Answer:

The correct answer is DNA profiling.

Explanation:

• DNA profiling is also known as DNA fingerprinting.
• Human genome contains certain regions called Microsatellite.
• These regions have sequence where there are multiple repeats of 2 to 16 base pair sequences (for example, ATGCATGCATGCATGC).
• The DNA polymerase's accuracy lowers while replicating these regions, due to multiple repeats.
• Hence, the copy number varies between individuals.
• The variation in copy number can be determined by PCR (polymerase chain reaction) amplification of these regions where the PCR fragment obtained will vary in their length depending upon the number of repeats.
• A child obtains two copies of DNA, each from his father and mother respectively.
• DNA profiling of the child's DNA will represent a pattern that would possess characteristics of both the parents.
• The biological parents of a child can hence be determined.