Justify the statement, agrobacterium tumefaciens is a good cloning vector
Answers
Agrobacterium tumefaciens is a good cloning vector :
Agrobacterium tumefaciens is used as a vector for plant transformation experiments.
It is used to transfer gene of interest into the plant. It is a good vector-
1. Carry selectable marker such as antibiotic resistance gene for identifying the recombinants.
2. Small in size.
3. Carry origin of replication so that recombinant DNA molecule can multiply in the host cells.
4. Contain multiple restriction sites for restriction enzymes.
5. It contains a tumour inducing plasmid(ti plasmid) in dicots and has been modified into a cloning vector.
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The following are the reasons for the Agrobacterium tumefaciens being a good cloning vector:
Explanation:
- The Agrobacterium tumefaciens has a tumor inducing plasmid also called as ti plasmid.
- This is a pathogen which introduces tumor in the dicot plants but it is disarmed to use it's plasmid.
- This pathogen yield plasmid of high copy number which can be used as a cloning vector.
- The vector can be introduced in other organisms to transfer desire gene of interest hence, very important for the purpose of genetic engineering.
Learn more about Agrobacterium tumefaciens:
Agrobacterium is a biofertilizer: https://brainly.in/question/6547483
What are AGROBACTERIUM TUMEFACIENS?: https://brainly.in/question/5915865