List and describe 5 major differences between culture-independent and culture-dependent microbiology?
Answers
Answer:
Culture-dependent approaches rely on growing prokaryotes. Culture- independent methods rely on molecular methods to study microbes within their environments.
Explanation:
Culture-independent diagnostic tests (CIDTs) are changing the way that clinical laboratories diagnose patients with foodborne illness. These tests can identify the general type of bacteria causing illness within hours, without having to culture, or grow the bacteria in a laboratory.
dependent:-
The primary advantage of culture-independent methods is their ability to readily identify a large proportion of the bacterial diversity that can be difficult to observe with culture-based studies.
Answer:
Culture-based approaches, while extremely useful for understanding the physiological potential of isolated organisms, do not necessarily provide comprehensive information on the composition of microbial communities (1, 46). Due to this documented disparity between cultivatable and in situ diversity, it is often difficult to assess the significance of cultured members in resident microbial communities. Recent studies, for example, have employed culture-independent molecular methods to show that cultivated microorganisms from both temperate and extreme environments often may represent very minor components of the microbial community (56, 61) as a whole.