Question

List the characteristics of an ideal vector to be used for gene cloning.

2

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Answer 1

Cʜᴀʀᴀᴄᴛᴇʀɪsᴛɪᴄs ᴏғ ᴀ ᴄʟᴏɴɪɴɢ ᴠᴇᴄᴛᴏʀs

• ɪᴛ ᴍᴜsᴛ ʙᴇ sᴍᴀʟʟ ɪɴ sɪᴢᴇ.
• Iᴛ ᴍᴜsᴛ ʙᴇ sᴇʟғ-ʀᴇᴘʟɪᴄᴀᴛɪɴɢ ɪɴsɪᴅᴇ ʜᴏsᴛ ᴄᴇʟʟ.
• Iᴛ ᴍᴜsᴛ ᴘᴏssᴇss ʀᴇsᴛʀɪᴄᴛɪᴏɴ sɪᴛᴇ ғᴏʀ Rᴇsᴛʀɪᴄᴛɪᴏɴ Eɴᴅᴏɴᴜᴄʟᴇᴀsᴇ ᴇɴᴢʏᴍᴇs.
• Iɴᴛʀᴏᴅᴜᴄᴛɪᴏɴ ᴏғ ᴅᴏɴᴏʀ DNA ғʀᴀɢᴍᴇɴᴛ ᴍᴜsᴛ ɴᴏᴛ ɪɴᴛᴇʀғᴇʀᴇ ᴡɪᴛʜ ʀᴇᴘʟɪᴄᴀᴛɪᴏɴ ᴘʀᴏᴘᴇʀᴛʏ ᴏғ ᴛʜᴇ ᴠᴇᴄᴛᴏʀ.

Answer 2

Answer:

cloning and Restriction enzymes....

Explanation:

Find out about historical overview of restriction enzymes, usage consideration, troubleshooting and non- cloning applications.

Cloning - Learn about the traditional cloning workflow, troubleshooting, PCR cloning, subcloning as well as library preparation and shotgun sequencing.

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