Question

List the steps of preparation of temporary mount of a leaf peel to observe stomata ?

Answer 1

The list of steps to be taken of preparation of temporary mount of a leaf peal to observe stomata are:

• To dissect a particular leaf from the potted plant.
• Carefully remove the peel from the ‘lower surface’ of the plant.
• Mount it on a glass slide to stain and view on the microscope.  
• Stain the peel with safranin.
• Keep a glycerol mount on the ‘slide and keep’ for observation under a microscope.

Answer 2

(i) The leaf peel of lower epidermis is taken and cut with a blade.

(ii) The cut pieces are transferred into a petridish containing water.

(iii) Leaf peel is stained with safranine.

(iv) Leaf peel after staining (2-3 minutes) is taken with a brush and kept on a clean glass slide.

(v) Mount the peel with glycerine and put coverslip on it.

(vi) Remove the excess stain and glycerin with the help of blotting paper.

(vii) Observe the slide under the microscope.