Question

Lumbrokinase for degradation and reduction of amyloid fibrils associated with amyloidosis

Answer 1

•Insulin amyloids are insoluble aggregates that develop due to chronic subcutaneous insulin injection in diabetic patients.
•Insulin is also a model protein for various amyloid associated diseases. These amyloids are harmful and their dissociation is warranted.
•In this study, Lumbrokinase was extracted from earthworm and found to be capable of partially degrading the insulin aggregates as evidenced by turbidity assay, ThT fluorescence, DLS and AFM analysis.
•The activity of Lumbrokinase was standardized and compared with a standard amyloid degrading enzyme Nattokinase.