Making competent cells and theory behind each step
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Hey there.!
As DNA is a hydrophilic molecule, it can't pass through the membranes.
they must be treated insuch a way that, bacteria takeup DNA on their own..let's see how this can be possible.
Inorder to make bacterial cells capable to take up DNA, they are treated with a divalent cation such as, Ca^+2.
This increases the efficiency with which DNA entera the bacterial cell.
rDNA can be sent into such type of cells by following certian steps:
- incubating the cells with DNA on ice.
- placing them briefly at 42°C (heat shock)
-Placing them back again on ice.
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HOPE THIS HELPS YOU
BE BRAINLY
As DNA is a hydrophilic molecule, it can't pass through the membranes.
they must be treated insuch a way that, bacteria takeup DNA on their own..let's see how this can be possible.
Inorder to make bacterial cells capable to take up DNA, they are treated with a divalent cation such as, Ca^+2.
This increases the efficiency with which DNA entera the bacterial cell.
rDNA can be sent into such type of cells by following certian steps:
- incubating the cells with DNA on ice.
- placing them briefly at 42°C (heat shock)
-Placing them back again on ice.
------------------------------------------------
HOPE THIS HELPS YOU
BE BRAINLY
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