Question

Mention any two functions of DNA polymerase.

Answer 1

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DNA polymerase I serve clean-up functions during replication, recombination, and repair. It has 3' to 5' proofreading exonuclease activity and 5' to 3' exonuclease activity. The 5' to 3' exonuclease activity of DNA polymerase I can replace an error containing a segment of DNA/RNA, paired to the template strand which is then simultaneously replaced by polymerase activity of the same enzyme. The process is called nick translation. DNA polymerase III is main DNA polymerising enzyme that has 5’ to 3’ polymerization and 3’ to 5’ exonuclease proofreading activities. PCR is a process of production of a large number of copies of a sample DNA using enzyme thermostable DNA polymerase. 

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