Mention the use of cloning vector in biotechnology?
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1.FOR transferring fragments of foreign DNA into suitable host.
2. To select recombinants from non-recombinants
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Cloning vectors are used to insert the recombinats DNA to get multiple copies of the desired genes
2. To select recombinants from non-recombinants
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Cloning vectors are used to insert the recombinats DNA to get multiple copies of the desired genes
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A cloning vector is DNA taken from a virus, plasmid or cells to be inserted with a foreign DNA fragment for cloning purposes. They often carry antibiotic resistance genes that can be used to test for expression of the plasmid DNA, on antibiotic petri plates. In other words, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmid, Lambda phages).
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