Question

Mention the use of tris buffer & EDTA during nucleic acid purification.

Answer 1

EDTA act as cation chelating agent. hence it is used in DNA isolation to chelate the Mg++ ions which are necessary in activity of nucleases so your DNA will be safe in presence of EDTA.

Tris-HCL act as stabilizing buffer during isolation of DNA, which maintain the pH of buffer.

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