What is affinity chromatography? Give its principle and applications
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Affinity chromatography is a method of separating biochemical mixture based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid.
The principle of affinity chromatography is as follows:
1) Inject a sample into an initially equilibrated affinity chromatography column.
2) Only the substances with affinity for the ligand are retained in the column.
3) Other substances with no affinity for the ligand are eluted from the column.
4) The substances retained in the column can be eluted from the column by changing pH or salt or organic solvent concentration of the eluent.
Affinity chromatography can be used in a number of applications, including nucleic acid purification, protein purification from cell free extracts, and purification from blood.
The principle of affinity chromatography is as follows:
1) Inject a sample into an initially equilibrated affinity chromatography column.
2) Only the substances with affinity for the ligand are retained in the column.
3) Other substances with no affinity for the ligand are eluted from the column.
4) The substances retained in the column can be eluted from the column by changing pH or salt or organic solvent concentration of the eluent.
Affinity chromatography can be used in a number of applications, including nucleic acid purification, protein purification from cell free extracts, and purification from blood.
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