Question

Molecular epidemiology of begomoviruses occurring on some vegetables, grain legume and weed species in the terai bel

Answer 1

Four vegetable crops (chilli, tomato, potato and sponge
gourd), one grain legume (black gram) and two weed species
(Ageratum conyzoides and Croton bonplandianum) showing
prominent symptoms of Begomovirus were sampled during
2011–2012 from the Bahraich district of the Terai belt in
northern India. Total genomic DNA isolated from two of each
infected samples were analyzed by PCR analysis using bego-
movirus coat protein gene specific primers. The amplified
products (~775 bp) were cloned and sequenced. Tomato leaf
curl New Delhi virus (ToLCNDV) was detected in sponge
gourd, chilli, potato and tomato; and Ageratum enation virus
(AEV) in black gram, A. conyzoides and C. bonplandianum
samples. In phylogenetic analysis, ToLCNDV, and AEV iso-
lates clustered in two distinct clades with their respective
begomovirus isolates. This is the first record of tentative
identification of AEV infecting black gram and C. bonplandi-
anum in India. The study also revealed the wide occurrence
of ToLCNDV in northern India

Answer 2

Explanation:

• Four vegetable crops (chilli, tomato, potato and sponge

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Croton
• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showing

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt in

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of each

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCR

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using bego

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using begomovirus coat protein gene specific primers. The amplified

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using begomovirus coat protein gene specific primers. The amplifiedproducts (~775 bp) were cloned and sequenced. Tomato leaf

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using begomovirus coat protein gene specific primers. The amplifiedproducts (~775 bp) were cloned and sequenced. Tomato leafcurl New Delhi virus (ToLCNDV) was detected in sponge

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using begomovirus coat protein gene specific primers. The amplifiedproducts (~775 bp) were cloned and sequenced. Tomato leafcurl New Delhi virus (ToLCNDV) was detected in spongegourd, chilli, potato and tomato; and Ageratum enation virus

• Four vegetable crops (chilli, tomato, potato and spongegourd), one grain legume (black gram) and two weed species(Ageratum conyzoides and Crotonbonplandianum) showingprominent symptoms of Begomovirus were sampled during2011-2012 from the Bahraich district of the Terai belt innorthern India. Total genomic DNA isolated from two of eachinfected samples were analyzed by PCRanalysis using begomovirus coat protein gene specific primers. The amplifiedproducts (~775 bp) were cloned and sequenced. Tomato leafcurl New Delhi virus (ToLCNDV) was detected in spongegourd, chilli, potato and tomato; and Ageratum enation virus(AEV) in black gram, A. conyzoides and C. bonplandianum