Question

name any 2 factors affecting hybridization

Answer 1

Temperature: If the temperature is too high, the strands melt. If it is too low, they might be forced together.

The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together.

The guanine to cytosine ratio (G:C ratio): Since this bond is stronger than the other nucleotide bonds, if the G:C ratio in the desired target strand is high, the separation process may take longer.