name any 2 factors affecting hybridization
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Temperature: If the temperature is too high, the strands melt. If it is too low, they might be forced together.
The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together.
The guanine to cytosine ratio (G:C ratio): Since this bond is stronger than the other nucleotide bonds, if the G:C ratio in the desired target strand is high, the separation process may take longer.
The pH: A pH that is too alkaline will cause the strands to separate; too acidic and they are forced together.
The guanine to cytosine ratio (G:C ratio): Since this bond is stronger than the other nucleotide bonds, if the G:C ratio in the desired target strand is high, the separation process may take longer.
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