Biology, asked by aggarwshiva200p5xtqy, 1 year ago

name any 4 methods of vectorless transfer of gene?


Anonymous: Is that frm biotech??
Anonymous: Do u need xplanation of each methd

Answers

Answered by mantasakasmani
3
Gene Transfer Methods:

The gene transfer techniques in plant genetic transformation are broadly grouped into two categories:

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I.    Vector-mediated gene transfer

II.    Direct or vector less DNA transfer

I. Vector-Mediated Gene Transfer:

Vector-mediated gene transfer is carried out either by Agrobacterium-mediated transformation or by use of plant viruses as vectors.

 II. Direct or Vector-less DNA Transfer:

The term direct or vector less transfer of DNA is used when the foreign DNA is directly introduced into the plant genome. Direct DNA transfer methods rely on the delivery of naked DNA into the plant cells. This is in contrast to the Agrobacterium or vector-mediated DNA transfer which may be regarded as indirect methods. Majority of the direct DNA transfer methods are simple and effective. And in fact, several transgenic plants have been developed by this approach.

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