Question

Name the two enzymes that are essential for constructing a recombinant DNA.

Answer 1

Answer:

Enzymes are DNA ligase, restriction endonucleases , DNA polymerase reverse transcriptase etc

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Answer 2

Restriction enzyme, DNA ligase

two enzymes essential for constructing recombinant DNA:

• restriction enzyme : in 1963 two enzymes responsible for restricting growth in E- coli were isolated , one of these added methyl group while the other cut DNA

• the latter one was extracted and the first restriction endonuclease hind II was prepared , works on a specific DNA nuleotide sequence (six base pairs) .

• the specific sequence was known as recognition sequence for hind II

• DNA ligase - the sticky ends produced on each strand helps in the action of DNA - ligase , and this helps the resultant DNA fragements to be joined together helping in formation of recombinant DNA molecule.