Need Interpretations of ELISA tests so that I can master ELISA Antibody titers ?
Answers
Answer:
I performed Indirect ELISA of 63 birds out of 63000 broiler poultry birds.
Age 33 days.
shed type : Semi control shed.
vaccinated against NDV was done at age 1 day old.
The mortality increased in the shed when ELISA performed by IDEXX kits by using Imark following was the result.
Need to discuss with experts.
Answer:
You found mean titer 10000+ for Shed 1 and 2 after 32 days of post vaccination. Though you did not mentioned type of vaccine like live or killed but it can interpreated as titer is good enough for protecting against NDV. In the same time CV% is showing slightly higher 55-57% that indicating titer is not homogenously distributed over the flocks. On the other hand Shed 4 is showing very low level titer and it is indicating birds are under risk of field infection. But for the proper interpretation of the report we should follow postmorterm lesions, clinical history and type of vaccination and baseline titer. It is suggested that you should test this sera for HI against local NDV. isolates.