Nucleic acid segment tagged with radioactive molecule is known as
(A) Clone
(B)
Probe
(C)
Plasmid
(D)
Vector
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Answer:
(B) Probe
Explanation:
DNA fingerprinting uses single stranded radioactive probes which are washed over the nylon surface to allow their joining to any DNA fragments of same composition thereby matching different DNA samples. Double stranded probes would not hybridize with DNA fragments present on the membrane and non radioactive probes can not be detected by autoradiogaphy. Autoradiography helps localizing these radioactively labelled molecules by applying photosensitive emulsion on the surface of the prepared slides
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