Question

optimum ph of enterokinase is acidic or basic?​

Answer 1

Answer:

Enterokinase cleavage is carried out at an enzyme/substrate ratio (w/w) of 1/20-1/200 for 1-24 h. For each fusion protein pilot experiments should be done to find out suitable conditions. The optimal pH for the reaction is between 7.0-8.0, but the enzyme can be used within pH 6.0-8.5.