organisation of plant tissue project works 9th class "big matter"
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Plant tissue culture is not a separate branch of plant science like taxonomy, cytology, plant physiology etc.
Rather it is a collection of experimental methods of growing large number of isolated cells or tissues under sterile and controlled conditions.
The cells or tissues are obtained from any part of the plant like stem, root, leaf etc. which are encouraged to produce more cells in culture and to express their totipotency (i.e. their genetic ability to produce more plants). Cells or tissues are grown in different types of glass vials containing a medium with mineral nutrients, vitamins and phytohormones. Therefore, to carry out the experiments using tissue culture techniques, a well-equipped laboratory is first required.
In recent years there has been a large increase in the number of research laboratories using tissue culture techniques to investigate many fundamental and applied aspects of higher plants. However, the use of these techniques is not confined to research alone.
Tissue culture techniques are being exploited by many commercial laboratories. Even many horticultural companies are setting up small units to multiply plants which are difficult to propagate by conventional means. The general organization of a tissue culture laboratory and the basic techniques will be discussed under different subheadings.
Tissue Culture Laboratory:
An ideal tissue culture laboratory should have at least two big rooms and a small room. One big room is for general laboratory work such as preparation of media, autoclaving, distillation of water etc. The other big room is for keeping cultures under controlled light, temperature and humidity. The small room is for aseptic work and for keeping autoclaved articles.
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Rather it is a collection of experimental methods of growing large number of isolated cells or tissues under sterile and controlled conditions.
The cells or tissues are obtained from any part of the plant like stem, root, leaf etc. which are encouraged to produce more cells in culture and to express their totipotency (i.e. their genetic ability to produce more plants). Cells or tissues are grown in different types of glass vials containing a medium with mineral nutrients, vitamins and phytohormones. Therefore, to carry out the experiments using tissue culture techniques, a well-equipped laboratory is first required.
In recent years there has been a large increase in the number of research laboratories using tissue culture techniques to investigate many fundamental and applied aspects of higher plants. However, the use of these techniques is not confined to research alone.
Tissue culture techniques are being exploited by many commercial laboratories. Even many horticultural companies are setting up small units to multiply plants which are difficult to propagate by conventional means. The general organization of a tissue culture laboratory and the basic techniques will be discussed under different subheadings.
Tissue Culture Laboratory:
An ideal tissue culture laboratory should have at least two big rooms and a small room. One big room is for general laboratory work such as preparation of media, autoclaving, distillation of water etc. The other big room is for keeping cultures under controlled light, temperature and humidity. The small room is for aseptic work and for keeping autoclaved articles.
______________________________
I hope I helped you
please mark as brainest
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