Pavani was doing experiments with flowers, buds, dry leaves and sprouting seeds. In which case respiration is maximum and minimum
Answers
Aim
To study and demonstrate the rate at which respiration takes place in flowering buds or in leaf tissue or in germinating seeds.
Theory
The rate of respiration can be estimated with the help of a Ganong’s respirometer as shown in the figure.
Diagram

Apparatus
The apparatus comprises three levelling parts of the tube:
For the respiring material, there is a bulb that terminates in a 10% KOH win small bulb at the base. The big bulb has a stopper with a lateral hole in it. It is through this hole that the atmospheric association is made through stopper regulation.
Graduated manometer embedded with the bulb.
A levelling tube fastened to the manometer using a rubber tubing. The complete set-up is secured on a stand.
Materials Required
10% KOH solution.
Ganong’s respirometer.
Germinating seeds or Flower petals or a Leaf Tissue.
Procedure
Measure 2ml of respiring material such as flower petals or germinating seeds and place it into the big bulb of the respirometer.
Into the manometer tube, add 10% KOH solution.
Initially, through regulation of the bulb stopper, atmospheric air around the respiring material is moved to the atmospheric pressure. The regulation continues till the neck of the bulb and the hole are coinciding.
The reservoir tube which is placed on the right is levelled in a way that the KOH solution inside the tube reaches the mark of 100ml at the base of the manometer.
Now the respiring material is enclosed by a setup of 100ml air.
To start the experiment, turn the glass stopper located at the top. This causes the atmospheric air to be cut off.
Observe the changes taking place.