Question

Phenol chloroform isoamyl alcohol extraction of mitochondrial dna

Answer 1

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Phenol/Chloroform – This is a mixture of buffer-saturated phenol and chloroform, usually close to 1:1 for DNA purification with other ratios sometimes used for RNA purification. Isoamyl alcohol is sometimes included as an anti-foaming agent, but is generally thought to be an inert and optional addition.