plant cells can be converted to protoplast by treating them
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Cellulase, because it will digest the plant cell wall.
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Protoplasts are basically plant cells that have been cleared out of their cell walls through the action of pectinases and cellulases. Plant cells can be converted into protoplast by direct delivery of DNA into the plant cells individually using polyethylene glycol or electroporation.
Protoplast transformation has efficiently highest frequency and has been used to successfully deliver genome-editing re-agents into multiple crop plants. But the technique is also labor intensive and time consuming and is not successful for limited plant species.
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