Question

plasmids of which bacteria were used to produced the Achain and B chain separately​

Answer 1

Answer:

Explanation:

we deliberately chose to construct two separate bacterial strains, one ... fragments coding for the A and B chains of human insulin were described ... Plasmids, including pBR322 (8), were isolated by a published procedure ... The procedure used to construct the A-chain gene was also ... (iv) The A chain produced by. E. coli ...

Answer 2

Answer:

E.coli

Explanation:

DNA sequences corresponding to A and B, Chains of human insulin and introduced them in plasmids of E.coli to produce polypeptide chains of insulin.

To minimize the complexity in the production of insulin by overcoming the process maturation of insulin by removal of C-peptide the Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin(active).