Please answers these questions
and please .
Answers
Answer:
a) 15N is the heavy isotope of nitrogen and it can be separated from 14N based on the difference in their densities.
b) 15N was incorporated into newly synthesized DNA.
c) The two molecules were distinguished by caseium chloride centrifugation in which these two are separated into two different bands at different positions based on their densities.
d) E.coli culture is taken at equal intervals to know the process of the experiment as generation time of E.coli is 20 minutes.
e) After 20 minutes the culture had an intermediate density showing a band in the middle tube and after 40 minutes, the culture had equal amounts of hybrid DNA and the light DNA showing two bands, one in the centre and at the bottom.
f) They concluded that DNA replication occurs by semiconservative method.