Question

Preparation of chitosan nanoparticles by ionic gelation

Answer 1

Chitosan nanoparticles were synthesized via the ionotropic gelation (25-27) of chitosan with TPP anions. Chitosan was dissolved in acetic aqueous solution at different concentrations (1, 2, 3 mg/mL). The concentration of acetic acid in aqueous solution was 1.5 time higher than that of chitosan (28). The TPP solution (1 mg/mL) was prepared by double-distilled water. Chitosan nanoparticles were spontaneously fabricated with the dropwise addition of 5 mL of the chitosan solution to 2 mL of TPP solution under magnetic stirring (1000 rpm, 1 hour) at room temperature. The opalescent suspension was formed under the same above mentioned conditions.