Question

Preparation of culture media for bacterial cultivation

Answer 1

Answer:

The method for the preparation of basic microbiology media is given below. In situations where preparation is uneconomic in time, prepared, sterilized media (liquid and solid) are available from the major school science equipment suppliers. Sterilization is at 121 °C (15 lb in ˉ²) for 15 minutes. pH values are 7.0 unless stated otherwise.

Note: Allow 15 cm³ of agar for each Petri dish and 5-10 cm³ of broth for each McCartney bottle. All cotton wool plugs should be made of non-absorbent cotton wool. Plastic or metal caps may also be used.

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