Question

preparation of human check cells and write it's AIM, material required procedure, conclusion precautions​

Answer 1

Answer:

To Prepare Stained Temporary Mount of Human Cheek Cells

Materials Required

Real Lab Procedure

• Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.
• Place the cells on a glass slide that has water on it.
• Mix the water and the cheek cells using a needle and spread them.
• Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.
• After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.
• Take a few drops of glycerine using a dropper and add this to the test mixture.
• Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
• Using a brush and needle, press the cover slip gently to spread the epithelial cells.
• Remove any extra liquid around the cover slip using a blotting paper.
• Place this glass side on the stage of the compound microscope and view it.

Observations

• A large number of flat and irregular-shaped cells are observed.
• The cells do not have a cell wall. However, each cell has a thin cell membrane.
• A deeply stained nucleus is observed at the centre of each cell.
• No prominent vacuoles are observed in the cells.

Conclusion

• As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.