Presence of selenocysteine in protein in
e.Coli is a consequence of
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Selenocysteine (Sec or U) is often referred to as the 21st amino acid. ... Moreover, formate dehydrogenase could be produced because it is native to E. coli and the elaborate machinery required for selenocysteine insertion is present in its gene.
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When cells are grown in the absence of selenium, translation of selenoproteins terminates at the UGA codon, resulting in a truncated, nonfunctional enzyme. The UGA codon is made to encode selenocysteine by the presence of a selenocysteine insertion sequence (SECIS) in the mRNA.
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