Question

Principle of chromatography

Answer 1

Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase. The sample components are separated into fractions based on their relative affinity towards the two phases during their travel.

The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.

I hope it will help you.

Answer 2

See, paper chromatography is based difference in absorption of constituents baby a surface of an appropriate adsorbent Material Or solid medium (I.e.stationary phase) .The rate of adsorption of a particular constitute depends upon its Solubility vin the solvent (Moving phase)



I hope it clarify your doubt and

Vande Mataram and Jay Hind.....