Principle of kjeldahl method of nitrogen estimation
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Principle Of Kjeldahl Method. In the digestion step, organic nitrogen is converted to an ammonium in the presence of a catalyst at approximately 370°C. In the distillation step, the digested sample is made alkaline with NaOH and the nitrogen is distilled off as NH3 . This NH3 is “trapped” in a boric acid solution.
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✔ In this method, the compound
heated with concentrated sulphuric acid in the presence of small amount of to convert nitrogen into Ammonium Sulphate
✔The contents of the flask are transferred to another flask and heated with excess of sodium hydroxide solution to liberate ammonia gas
✔ Ammonia gas so liberated is passed and absorbed known as the volume concentrated sulphuric acid
✔Organic compound +
✔