Question

procedure of fish cartilage

Answer 1

We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 microns in length can be clearly visualized. The method is suitable for both ontogenic studies during early stages of skeletal development in most marine fishes (e.g., Sparus aurata L., Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities in small larvae. This procedure can also be used for specimens that have been preserved in 100% ethanol for up to two years.

Answer 2

Cartilaginous fish (scientific name Chondrichthyes) have a skeleton made from cartilage instead of bone. Cartilage is tough and flexible and provides enough structural support to allow these fish to grow to very large sizes. Cartilaginous fish include sharks, rays, skates and chimaeras.

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