Q.1. This experiment investigates the effect of Neutrase on the breaking of a protein in milk called casein Background: Powdered milk contains a protein called casein. When powdered milk is dissolved in water to make a solution, this protein in suspension makes the solution opaque (you cant see through it), in this investigation you will add different concentations of a protease enzyme (break down protein) called neutrase to a powdered milk solution and time how long it takes for the milk solution to clear. Materials: • 5% neutrase solution • 2% powdered milk solution • 5 ml syring, 50 ml measuring cylinder • 3X 100 ml beakers • Stop clock • Paper with pencil crosses marked on Method: 1. Work in pairs, put on your safety glasses. 2. Place a piece of paper with a cross drawn on it on the bench and put a 100 ml beaker on top. 3. Using a 5 ml syring add 5ml of 5% neutrase solution to the beaker. 4. Using a measuring cylinder add 25 ml of 2% powdered milk solution ot the beaker. Pour the milk rapidly ino the beaker to ensure the milk and neutrase mix well. 5. As soon as you have added the milk solution start the stop clock. 6. Looking from above, stop the clock at the moment you can see the cross through the milk. 7. Record the time taken in your table. 8. Using the information in the table opposite make up the other concentratios of neutrase solution (4%, 3%, 2% and 1%) and repeat the experiement at each different enzyme concentration. 9. Repeat the entire experiemnt twice again (at all fivr enzyme concentrations) Created by: Dr. Archana Gaikwad (A) State the three variables (B) What were the values of your dependent variable? (C) Formulate and explain a testable hypothesis using correct reasoning (D) Accurately interpret the data and explain your results using correct scientific reasoning. (E) Evaluate the validity of the hypothesis based on the outcome of the investigation (F) Evaluate the validity of the method based on the outcome of the investigation (G) What was the main hazard in the experiment and how did you eliminate this hazard? (H) Explain improvements or extensions to the method that would benefit the investigation. Once you have correctly collected, organized, transformed and presented your data in a table, plot a graph of your mean results. Make sure you have: • suitable title • suitable scale on both axes • both axes labelled (including units) • points accurately plotted • points joined together in the most suitable man
Answers
Answer:
Independent variable- Amount of neutrase solution
dependent variable- time taken for reaction
Controlled variable- concentration of powdered milk solution
Research Question- -How does the concentration of neutrase solution affect the time it takes for the solution to turn opaque?
Hypothesis- If the concentration is higher, the solution will break down faster as it will break down proteins faster compared to low enzyme concentration.
D) As given in the above data, it is concluded that the neutrase and powdered milk solution are inversely related. As the concentration of enzymes increase, theres more break down of proteins than without enzymes, therefore decreasing the time for it to clear.
E)The method is valid because it contains all the necessary measurements and materials required for the experiment.
F)A high chance of solution is possible because of the enzyme reaction, so I used safety glass and maintain a distance from the breaker
G)Try increasing the concentration of milk solution and check if the results are similar
(couldn't add the other two answers sowwy ._.