Q.2 During heat shock to the bacterium, t
temperature used for giving thermal shock
Options:
(a) 52°C (b) 100°C
(c) liquid nitrogen (d) 42°C
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Answer: option d
Heat Shock is given to bacteria for transformation that alters the membrane fluidity. The increase in temperature creates pores in the bacterial plasma membrane and allow plasmid DNA to enter the bacterial DNA. For the heat shock, the DNA-bacteria mixture is put into a 42C to 37C water bath
Heat Shock is given to bacteria for transformation that alters the membrane fluidity. The increase in temperature creates pores in the bacterial plasma membrane and allow plasmid DNA to enter the bacterial DNA. For the heat shock, the DNA-bacteria mixture is put into a 42C to 37C water bath
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