Question

Q.2.Shyam being very inquisitive, didn’t listen to the teacher’s instruction properly. He took the thin layer of onion peel with a drop of water on the glass slide. He placed the coverslip on it and start observing the slide under a microscope. But he was unable to see the cells of onion peel properly. • What do you think he might have forgotten? • What is the function of a dye? • What values do you get from the above incidence

Answer 1

Answer:

Transparent membranous structure of the onion should be peeled off with the help of forceps. Add the peel in water. With the help of blotting paper remove the excess of water. Remove the extra glycerine or oil with the help of blotting paper.

Answer 2

A Wet Mount Slide is prepared to perform the activity of observing the onion peel under a microscope.

Explanation:

• Shyam has forgotten to stain the onion peel which helps to differentiate its cells. Due to lack of staining, he is not able to observe the onion peels.
• The function of dying/staining of cell is to make it easier to see the cell or specific components. Staining cells is sometimes done to highlight metabolic processes or to distinguish between live and dead cells.
• The moral value that we incur from the given incidence is: Following the instructions of Teachers carefully and Paying attention to them properly while working in lab.