Q.28 The step NOT involved to
produce recombinant DNA using
human DNA?
1insertion of DNA in to vector
2transformation of the cell using
vector
3isolate and purify dna
4use of retroviruses to circularise
dna
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The step which is NOT involved to produce a recombinant DNA (rDNA) using human DNA is use of retrovirus to circularize DNA.
What is recombinant DNA technology?
It is an invitro biotechnological method employed to manipulate the genetic material of an organism to create new genetic combinations with new functions. It is also known as genetic engineering.
The process of recombinant DNA technology involves:
- Isolation of gene of interest or DNA from the organism.
- Insertion of this DNA into a suitable vector or plasmid which acts as a carrier forming recombinant DNA (rDNA) molecule.
- Introduction of vector into the cell generally bacterial cell which produces multiple copies.
- Isolation of these multiple copies of DNA and its purification.
To learn more about recombinant DNA process refer:
https://brainly.in/question/7305107
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