Question

Q. Staining a bacterial smear by gram staining method. describe the gram staining Method?

Answer 1

✌️✌️Hello sir!!!!!

the gram staining Method:

Bacterial smear
⬇️
Air Drying
⬇️
stained with crystal violet/ gentian Violet (for 30 seconds)
⬇️
Bacteria get stained bluish purple
⬇️
washed with water
⬇️
Iodine staining (0.5% solution) (iodine works as mordant)
⬇️
formation of crystal violet iodine complex
⬇️
treatment with alcohol
⬇️ ⬇️
some bacteria Destined bacteria
retain the. ⬇️
stain Counter staining with
⬇️ safranin
gram ⬇️
positive pink colour appears
bacteria ⬇️
gram negative bacteria


thanks.....
nice to help you ✌️✌️

Answer 2

Thanks for the question!

It is definitely a very interesting question to solve and do some brainstorming.

**************************************************

Staining is an auxiliary technique  used in microscopic techniques. It is extensively used to enhance the clarity of the microscopic image.

Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives.

The four basic steps of the Gram Stain are:

1) Application of the primary stain Crystal Violet (CV) to a heat-fixed smear of bacterial culture.

2) Addition of Gram’s Iodine.

3) Decolorization with 95% ethyl alcohol.

4) Counterstain with Safranin

Conclusion:

1. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall.

2. Gram negative bacteria: Stain red or pink due to retaining the counter staining dye called Safranin.

**************************************************

Hope it helps and solves your query!!