Qualitative analysis of chromosomes from neural ganglia of flesh-flies sarcopahga
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Explanation:
A simplified technique has been developed in order to obtain and visualize large number of mitotic chromosomes at metaphase stage in the neural ganglial cells to facilitate the detection of various banding patterns which play a significant role in cytogenetical analysis of flesh-flies. Several studies have been performed on the flesh-fly Sarcophaga related to their role in forensics, as vectors of pathogenic micro-organisms, in the isolation of many useful proteins, studies related to circadian cycle, developmental and metabolic processes which require enough time as well as a good infrastructure; yet basic cytogenetical methods remain the prerequisite for perusing genetical analysis. In view of this fact, the present study was conducted to determine a simple, rapid and qualitative method that is indeed helpful in the cytogenetical studies of Sarcophaga species as well as other dipterans even without the requirement of any special equipment.
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