Question

Quantitative measure of a columns ability to separate two solutes is called_______.

Answer 1

Answer:

Partition Chromatography

Answer 2

Answer:

The Resolution is a correct answer.

Explanation:

• Quantitative measure of a column's ability to separate two solutes is called Resolution (Rs).
• If we consider chromatograms for species A & B on 3 columns with different resolving powers, then the resolution of each column is defined with the equation given below in the picture.
• Also, from the second picture (chromatogram) given below, it is evident that a resolution of 1.5 provides complete separation of A & B but a resolution of 0.75 does not.

1. Zone A consists of 4 percent B at a resolution of 1.0.
2. Overlap of approx. 0.3 percent is seen at a resolution of 1.5.
3. By increasing the number of plates and stretching the column the resolution of a given stationary phase can be improved.
4. Due to the addition of plates time required for separating the components will be increased.