Question

Restriction endonuclease hydrolyzes polynucleotide
from
(1) Only the 5' end
(2) From either terminal
(3) At any internal phosphodiester bond
(4) A phosphodiester bond with in a specific
sequence​

Answer 1

The correct option is 4) A phosphodiester bond with in a specific  sequence​.

Explanation:

• Restriction endonucleases can be described as the enzymes that are involved in cutting of the DNA from a specific site within a molecule.
• Restriction enzymes cleave foreign DNA, thus eliminating infecting organisms naturally.
• This phenomenon is used in biotechnology for genetic engineering,
• Other options like option 3 is not correct because restriction enzymes are specific in their function.

Answer 2

(4) A phosphodiester bond with in a specific sequence