Restriction endonucleases have played a very significant role in rDNA technology. Explain the roles of EcoRI and DNA ligase in formation of recombinant DNA.
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DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids
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