Question

selection of pBR322 using antibiotic resistance​

Answer 1

Explanation:

ampicillin resistance

The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez." pBR322 is 4361 base pairs in length and has two antibiotic resistance genes – the gene bla encoding the ampicillin resistance (AmpR) protein, and the gene tetA encoding the tetracycline resistance (TetR) protein.

Answer 2

Answer:

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selection of pBR322 using antibiotic resistance_??

$\underline \mathscr \purple{Answer \: : - }$

Selection using Antibiotic resistance: The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinant ones by plating the transformant on tetracycline-containing medium The transformant growing on ampicillin-containing medium then transferred on a medium containing tetracycline. The recombinants will grow in ampicillin-containing medium but not on that containing tetracycline. But, non-recombinants will grow on the medium containing both the antibiotics. In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance gene gets "inactivated due to insertion" of alien DNA and helps in selection of recombinants.