Question

Short note on pulse chase

Answer 1

⛦ Hᴇʀᴇ Is Yoᴜʀ Aɴsᴡᴇʀ⚑
▬▬▬▬▬▬▬▬▬▬▬▬▬☟

Pulse chase➙ An experiment in which cells are grown in radioactive medium for a brief period (Pulse) and then transferred to nonradioactive medium for a longer period (Chase).

________________________⤵


❖ It is a method for examining a cellular process occurring over time by successively exposing the cells.

❖ It use labeled compounds to follow the dynamics of cellular processes & pathways.

❖ During the pulse phase of the experiment, cells are exposed to a labeled compound.

❖ Various protein labeling methods for pulse-chase have specific application potentials.

❖ It often used to study the degradation of macromolecules such as proteins or mRNA.

❖ It is a key technique in biochemistry and cell biology.

____________
Thanks...☕✊

Answer 2

${\fbox{\fbox{\fbox{\huge\sf\pink{AnsWeR}}}}}$

➮ ᴘᴜʟsᴇ-ᴄʜᴀsᴇ ᴀɴᴀʟʏsɪs ɪs ᴀ ᴍᴇᴛʜᴏᴅ ғᴏʀ ᴇxᴀᴍɪɴɪɴɢ ᴀ ᴄᴇʟʟᴜʟᴀʀ ᴘʀᴏᴄᴇss ᴏᴄᴄᴜʀʀɪɴɢ ᴏᴠᴇʀ ᴛɪᴍᴇ ʙʏ sᴜᴄᴄᴇssɪᴠᴇʟʏ ᴇxᴘᴏsɪɴɢ ᴛʜᴇ ᴄᴇʟʟs ᴛᴏ ᴀ ʟᴀʙᴇʟᴇᴅ ᴄᴏᴍᴘᴏᴜɴᴅ (ᴘᴜʟsᴇ) ᴀɴᴅ ᴛʜᴇɴ ᴛᴏ ᴛʜᴇ sᴀᴍᴇ ᴄᴏᴍᴘᴏᴜɴᴅ ɪɴ ᴀɴ ᴜɴʟᴀʙᴇʟᴇᴅ ғᴏʀᴍ (ᴄʜᴀsᴇ).

☞ɪɴ ᴀ ᴘᴜʟsᴇ ᴄʜᴀsᴇ ᴇxᴘᴇʀɪᴍᴇɴᴛ, ᴄᴇʟʟs ᴀʀᴇ ɢʀᴏᴡɴ ɪɴ ᴍᴇᴅɪᴜᴍ ᴀɴᴅ ᴇxᴘᴏsᴇᴅ ғᴏʀ ᴀ sʜᴏʀᴛ ᴛɪᴍᴇ ᴘᴇʀɪᴏᴅ ᴛᴏ ᴀ ʟᴀʀɢᴇ ᴀᴍᴏᴜɴᴛ ᴏғ ʀᴀᴅɪᴏᴀᴄᴛɪᴠᴇ ᴀᴍɪɴᴏ ᴀᴄɪᴅ. ᴛʜɪs ɪs ᴄᴀʟʟᴇᴅ ᴛʜᴇ ᴘᴜʟsᴇ, ᴀɴᴅ ᴅᴜʀɪɴɢ ᴛʜɪs ᴛɪᴍᴇ ᴘᴇʀɪᴏᴅ, ᴀɴʏ ɴᴇᴡʟʏ sʏɴᴛʜᴇsɪᴢᴇᴅ ᴘʀᴏᴛᴇɪɴs ᴡɪʟʟ ɪɴᴄᴏʀᴘᴏʀᴀᴛᴇ ᴛʜᴇ ʀᴀᴅɪᴏᴀᴄᴛɪᴠᴇʟʏ ᴀᴍɪɴᴏ ᴀᴄɪᴅ.