Short note on shoot multiplication
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The objective of the shoot multiplication stage is to increase the number of shoots produced by the original explant. By subculturing these new shoots on to new medium, the number of shoots produced in culture increases dramatically. The technician is preparing to subculture.
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The established explantsare subcultured after 2-3 weeks, on shootmultiplication medium. The medium is designedin such a way to avoid the formation of callus,
which is undesirablefortrue
totypemultiplication of plants. Thus, careful use of auxins like NAA, 2,4-D and cytokininslike BAP, kinetin is done in culture medium. It is well-established fact that cytokininsenhance shoot multiplication.
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